Date:
Wed, 23/06/202110:00-11:30
Location:
Danciger B Building, Seminar room
Lecturer: Oleg Krichevsky - Ben Gurion University of the Negev
Abstract:
Hematopoietic stem cells (HSCs) are the progenitors of all of the immune cells in the body. They reside in the bone marrow (BM) but can be mobilized into blood for use in transplantation. In homeostasis, HSCs were thought to stay static within their BM niches. We used two-photon laser scanning microscopy to visualize genetically labeled HSCs in the BM of live mice for several hours. The majority of HSCs showed a dynamic non-spherical morphology and significant motility, undergoing slow processive motion interrupted by short stretches of confined motion. Interestingly, mobilization-inducing drug treatment rapidly abrogated HSC motility and shape dynamics in real time. Our results reveal an unexpectedly dynamic nature of HSC residence in the bone marrow.
Abstract:
Hematopoietic stem cells (HSCs) are the progenitors of all of the immune cells in the body. They reside in the bone marrow (BM) but can be mobilized into blood for use in transplantation. In homeostasis, HSCs were thought to stay static within their BM niches. We used two-photon laser scanning microscopy to visualize genetically labeled HSCs in the BM of live mice for several hours. The majority of HSCs showed a dynamic non-spherical morphology and significant motility, undergoing slow processive motion interrupted by short stretches of confined motion. Interestingly, mobilization-inducing drug treatment rapidly abrogated HSC motility and shape dynamics in real time. Our results reveal an unexpectedly dynamic nature of HSC residence in the bone marrow.